Composite
Part:BBa_K5520012:Design
Designed by: Ruyi Shi Group: iGEM24_Ulink-SZ (2024-09-29)
pT7-LacO-His- CsnBV186Y
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 844
Illegal NotI site found at 804 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 813
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 153
Illegal AgeI site found at 636 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The objective of the study was to elucidate the structural-functional relationship of chitosanase and to facilitate the production of chitooligosaccharides with a precise degree of polymerization. To this end, the amino acid valine at position 186 was substituted with tyrosine to enhance enzymatic activity and to produce more homogeneous products.
Source
Escherichia coli and Bacillus pumilus